ASU SoLS:DNA Laboratory - Services and Fees

Single Pass Sequencing:
For ASU affiliated customers:
$4/ sequencing reaction for PCR or plasmid, $5/BAC or cosmid.
$3.50/sequencing reaction in a 48 or 96 well plate or tube strips.
$1.00 for those who prefer to run and purify reactions themselves.

For non–ASU affiated customers:
$8.25/sequencing reaction for PCR or plasmid.
$7.50/sequencing reaction in a 48 or 96 well plate or tube strips.
$1.50 for those who prefer to run and purify reactions themselves.

Our rerun policy. We’ll rerun at no cost any sample giving suboptimal sequence, for whatever reason. Just let us know on the sheet that this is a repeat. We can often help if sequencing isn’t going as well as you’d like. Talk to us.

Special conditions: If you have a high GC content or known secondary structure, let us know on your sheet. We can alter our reactions for you. We offer more primers than are listed on the submission form. Check the link at the bottom of the form.

Fragment Analysis: $26/plate of 96. for ASU and affiliated customers: Non–ASU call for pricing.

Plasmid Preparations: $130/96 well plate; $2.00 each for individual samples. Individual samples done only when accompanying a sequencing order.

PCR Purifications: $75/96 well plate, 37.50/48 samples; $1.25 each for individual samples. Done only when accompanying a sequencing order.

Microarray Scanning: $10/slide ASU and affiliates; non ASU call for pricing.. Please call about other microarray procedures.

Bioanalyzer RNA Runs: $21/run ASU and affiliates: (12 samples); non–ASU call for pricing. Please call about running other chip types.

Real time PCR: $12/run ASU and affiliates, Including plate and optical cover; non–ASU call for pricing.

Purity and quantitation of the DNA template and primer dictate the quality of the sequencing results. The templates should be purified and quantified before submitting for sequencing. We can quantify templates for you, especially off campus customers; however this may delay the return of your data somewhat.

The optimal amounts for templates and primers are as follows:

The amount of primer required for all of the above is 20 ng, except BAC and Cosmid which need 100 ng primer

Mix the template and primer at above amount and bring the volume to 6 ul with dd H2O. For off campus customers we recommend drying the sample prior to shipment to avoid possible loss.

Sequencing Results:
We routinely deliver data by email. For large projects a disk can be delivered.
Text files open with word or notepad. To open electropherogram files use the programs at the following links:

Fragment analysis Results:
We can deliver data by email or by disk. To open the data files use programs at one of the following links: