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RICHARD N. TRELEASE Professor Ph.D., University of Texas Phone: (480) 965-2669 Send e-mail to trelease.dick@asu.edu

Dr. Trelease received his Ph.D. degree from the University of Texas, Austin. I came to Arizona State University after doing research as a NIH postdoctoral fellow at the University of Wisconsin, Madison. Main activities include serving on numerous research proposal panels for the National Science Foundation in the Division of Molecular and Cellular Biosciences, chairing Graduate Studies Committees for Plant Biology and later for the Molecular and Cellular Biology (MCB) Program, and achieving consideration as a finalist for two different years for the ASU College of Liberal Arts and Sciences Quality Teaching Award. Active participation in helping form the new School of Life Sciences (SoLS) in 2003-2004 included searches for new faculty in the field of Molecular Interactions/Bioimaging and representation on the SoLS Strategic Planning Committee.

Research in my laboratory is focused in the areas of intracellular protein trafficking and organelle biogenesis in plant cells. More specifically, experiments are aimed at discovering the intracellular sorting pathways and deciphering of necessary and sufficient molecular targeting signals for newly synthesized membrane proteins that end up in plant peroxisomes. Two major types of peroxisomal membrane proteins (PMPs) are being examined in graduate student and postdoctoral fellow research projects. One type is the so-called constitutive maintenance PMPs such as transporters, antioxidant enzymes, receptors, etc., and the other type includes PMPs, named peroxins, that participate in the biogenesis (proliferation, differentiation) of peroxisomes.

A majority of our studies are conducted with model cell systems, namely suspension-cultured BY-2 and Arabidopsis cells, rather than with whole plants. Most of the genes selected for study code for Arabidopsis homologs with an ultimate goal of determining their biogenetic or metabolic function(s).

Experimental approaches involve confocal immunofluorescence microscopy, recombinant DNA technology, PCR mutagenesis, cell fractionation, biochemical assays, immunogold electron microscopy, etc. Research in my laboratory is strongly graduate student oriented; students often work cooperatively with postdoctoral scientists in the lab as evidenced by the following recent publications.

Selected Publications

Karnik SK and RN Trelease (2007) Arabidopsis Peroxin 16 trafficks through the ER and an intermediate compartment to pre-existing peroxisomes via overlapping molecular targeting signals. J Experimental Bot 58:1677-1693.

Mullen RT and RN Trelease (2006) The ER-peroxisome connection in plants: development of the "ER semi-autonomous peroxisome maturation and replication" model for plant peroxisome biogenesis. Biochim Biophys Acta - Molecular Cell Research. 1763:1655-1668.

Trelease RN and MJ Lingard (2006) Participation of the plant ER in peroxisomal biogenesis. In: DG Robinson (Ed.), The Plant Endoplasmic Reticulum, Plant Cell Monographs 4. Springer-Verlag, Heidelberg Germany (2006) pp 205-232.

Lingard MJ and RN Trelease (2006) Five Arabidopsis peroxin 11 homologs individually promote peroxisome elongation, division without elongation, or aggregation. Jour Cell Sci: 119:1961-1972.

Lisenbee, CS, MJ Lingard, and RN Trelease (2005) Arabidopsis peroxisomes possess functionally redundant membrane and matrix isoforms of monodehydroascorbate reductase. The Plant Journal 43:900-914.

Karnik, SK and RN Trelease (2005) Arabidopsis peroxin 16 coexists at steady state in peroxisomes and endoplasmic reticulum. Plant Physiol 138:1967-1983.

Flynn, CR, M Heinze, U Schumann, C Gietl, and RN Trelease (2005) Compartmentation of the plant peroxin, AtPex10p, within subdomain(s) of ER. Plant Science 168:635-652.

Leivar, P, VM Gonzalez, S Castel, RN Trelease, et al. (2005) Subcellular localization of Arabidopsis 3-hydroxy-3-metylglutaryl-Coenzyme A reductase. Plant Physiol 137:57-69.

Hunt, J and RN Trelease (2004) Sorting pathway and molecular targeting signals for the Arabidopsis peroxin 3. Biochem Biophys Res Comm 314:586-596.

Lisenbee, CS, M Heinze, and RN Trelease (2003) Peroxisomal ascorbate peroxidase resides within a subdomain of rough endoplasmic reticulum in wildtype Arabidopsis cells. Plant Physiol 132:870-882.

Lisenbee, CS, SK Karnik, and RN Trelease (2003) Overexpression and mislocalization of a tail-anchored GFP redefines the identity of peroxisomal ER. Traffic 4:491-501.

Trelease, RN (2002) Peroxisomal Biogenesis and Acquisition of Membrane Proteins, Chapter 10, In: PLANT PEROXISOMES, A.Baker and Graham, eds., Kluwer Academic Publishers, Dordrecht, The Netherlands. pp 335-338.

Mullen, RT, CS Lisenbee, CR Flynn, and RN Trelease (2001) Stable and transient expression of chimeric peroxisomal membrane proteins induces and independent zippering of peroxisomes and an ER subdomain. Planta 213:849-863.

Mullen, RT, CR Flynn, and RN Trelease (2001) How are peroxisomes formed? The role of the endoplasmic reticulum and peroxins. Trends in Plant Science 6:256-261.

Corpas, FJ, LM Sandalio, MJ Brown, LA del Rio, and RN Trelease (2000) Identification of porin-like polypeptide(s) in the boundary membrane of oilseed glyoxysomes. Plant Cell Physiol. 41(11):1218-1228.

Mullen, RT and RN Trelease (2000) The sorting signals for peroxisomal membrane-bound ascorbate peroxidase are within its carboxy-terminal tail. J Biol Chem 275:16337-16344.

Mullen, RT, CS Lisenbee, JA Miernyk, and RN Trelease (1999) Peroxisomal membrane ascorbate peroxidase is sorted to a membranous network that resembles a subdomain of the endoplasmic reticulum. The Plant Cell 11:2167-2185.

Corpas, FJ, and RN Trelease (1998) Differential expression of ascorbate peroxidase and a putative molecular chaperone in the boundary membrane of differentiating cucumber seedling peroxisomes. J Plant Physiol 153:332-338.

Flynn, CR, RT Mullen, and RN Trelease (1998) Mutational analyses of a type 2 peroxisomal targeting signal that is capable of directing oligomeric protein import in tobacco BY-2 glyoxysomes. The Plant Journal 16:709-720.

Corpas, FJ, LM Sandalio, L del Rio, and RN Trelease (1998) Copper-zinc superoxide dismutase is a constituent enzyme of the matrix of peroxisomes in the cotyledons of oilseed plants. New Phytol 138:307-314.

Corpas, FJ, and RN Trelease (1997) The plant 73 kDa peroxisomal membrane protein (PMP73) is immunorelated to molecular chaperones. Europ J Cell Biol 73:49-57.

Lee, MS, RT Mullen, and RN Trelease (1997) Oilseed isocitrate lyases lacking their essential type 1 peroxisome targeting signal are piggybacked to glyoxysomes. The Plant Cell 9:185-197.

Mullen, RT, MS Lee, and RN Trelease (1997) Identification of the peroxisomal targeting signal for cottonseed catalase. The Plant Journal 12:313-322.

Mullen, RT, MS Lee, CR Flynn, and RN Trelease (1997) Diverse amino acid residues function within the type 1 peroxisomal targeting signal (PTS1). Implications for the role of accessory residues upstream of the PTS1. Plant Physiol 115:881-889.

Bunkelmann, J, and RN Trelease (1996) Ascorbate peroxidase: A prominent membrane protein in oilseed glyoxysomes. Plant Physiol 110:589-598.

Trelease, RN, MS Lee, A Banjoko, and J Bunkelmann (1996) C-terminal polypeptides are necessary and sufficient for in-vivo targeting of transiently-expressed proteins to peroxisomes in suspension-cultured cells. Protoplasma 195:156-167

Mullen, RT, and RN Trelease (1996) Biogenesis and membrane properties of peroxisomes: does the boundary membrane serve and protect? Trends Plant Sci 1(11):389-394.