EM Laboratory
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Equipment
Instruments
Electron microscopes in the laboratory include:
- Philips CM 12 Scanning Transmission EM fitted with a Gatan model 791 CCD camera for direct digital image acquisition
- JEOL 1200EX TEM fitted with a SIA L3C digital camera
- Leica Cambridge 360FE scanning electron microscope, a gift from the Motorola Corp. This instrument is fitted with an IXRF digital imaging system
Supporting Equipment:
- Bal-Tec HPM 010 High Pressure freezer
- Bal-Tec FSU 010 freeze substitution unit
- Balzers model 400D freeze fracture system
- RMC MT 6000 ultramicrotome with CR 2000 cryosectioning attachment
- Leica Ultracut R ultramicrotome
- Reichert Ultracut E ultramicrotome
- Technics Hummer II sputter coater
- Balzers critical point dryer
- Ted Pella Inc. Microwave specimen preparation system
- Denton TSC tabletop carbon evaporator/sputter coater
- Harrick Plasma Cleaner/glow discharge unit
- Plus all necessary and basic lab instrumentation and equipment such as ovens, microfuge, pH meter, rotators, etc.
Facilities and Equipment
The Laboratory suite is purpose built for electron microscopy, with approximately 1400 square feet of floor space. There are two darkrooms, one for printing and one for processing of film. The printing darkroom is equipped with a floor standing Durst Model S–45 EM enlarger fitted with a point light source and capable of printing all formats 4"x5" or smaller plus developing tanks for processing sheet film, large sink for tray development of prints to 16" x 20" and an Ilford 1050 infrared print dryer. The film only darkroom has facilities for processing all formats of film from 4"x 5" down and includes a nitrogen burst agitation system for sheet film as well as film desiccators for TEM films and a film dryer. The preparation laboratory is equipped with a fume hood, the ultramicrotomy rooms with both dissecting and compound microscopes and fiber optic light sources, and the printing darkroom with a Thomas Super Safelight. There is also a room dedicated to high pressure freezing and graphics preparation.
Equipment Sign–Up Calendar
Qualified users of the LSEMF can reserve time on instruments or reserve darkroom time by going to the LSEMF Calendar at http://calendar.yahoo.com/. The password for the calendar can be obtained from David Lowry at dlowry@asu.edu. At the Yahoo site, type in the ID "ASULSEMF" and the password.
The user should include their name, the instrument they wish to use, and their desired beginning and ending times on the day they are requesting to use the facility. If someone is already signed up in the time slot for a DIFFERENT instrument, the user can sign up in the same time slot for another instrument and the calendar will make room for their request. It is considered bad manners to erase a sign up posted by another user. Please confine sign ups for any particular instrument to no more than three half day sessions per week.