Yung Chang
Research
My lab has been interested in the mechanisms underlying the formation and progression of lymphoid malignancy, such as leukemia and lymphoma. The oncogenic transformation of some lymphoid cells has been linked to an essential physiological process of lymphocyte development, V(D)J recombination. This recombination event proceeds through two steps: a site-specific cleavage to generate double stranded DNA breaks and joining of these breaks by ubiquitous DNA repair machinery. Defects in the recombination process could lead to immune deficiency and lymphoid malignancy. We have created in vitro cellular models that mimic some aspects of normal lymphocyte development, i.e., activation of V(D)J recombination and induction of lymphocyte differentiation. Recently, we demonstrated that a non-malignant pre-leukemia cell line could be induced to become leukemia cells. We have been exploring this inducible leukemia model to characterize factors and processes critical to the leukemia formation, and ultimately to identify biomarkers and targets for possible therapeutic intervention.
Selected Publications
Hahn, K. B. Beres, M. Rowton, M. Skinner, Y. Chang, A. Rawls, J. Wilson-Rawls. 2008. A deficiency of Lunatic fringe is associated with defects of the rete testis. Reproduction. In press.
Pavlicek, J, Y. Lyubchenko and Y. Chang. 2008. Revelation of RAG-induced DNA bending by atomic force microscopy. Biochemistry. 47:11204-11.
Jentarra, GM, M.C. Heck, J.W. Youn, K. Kibler, J.O. Langland, C. R. Baskin, O. Ananieva, Y. Chang & Jacobs BL. 2008. Vaccinia viruses with mutations in the E3L gene as potential replication-competent, attenuated vaccines: scarification vaccination. Vaccine. 26:2860-72.
Ke, Y., S. Lindsay, Y. Chang, Y. Liu and H. Yan. 2008. Self-assembled Water-soluble Nanoarrays for Label Free RNA Hybridization Assays. Science 319:180-183.
Wang, Q., Y. Xu, X. Zhao, Y. Chang, Y. Liu, L. Jiang, J. Sharma, D. K. Seo & H. Yan. 2007. A facile one-step in situ functionalization of quantum dots with preserved photoluminescence for bioconjugation. J. Am. Chem. Soc., 129:6380-6381.
Li, Z., Y. Chang. 2007. V(D)J recombination in zebrafish: normal joining products with accumulation of unresolved coding ends and deleted signal ends. Mol. Immunol. 44:1804-1813.
Zhong, H., Z. Li, S. Lin and Y. Chang. 2007. Initiation of V(D)J recombination in zebrafish (Danio rerio) ovaries. Mol. Immunol. 44:1795-1803.
Jacobsen, E. O. Ananieva, M. L. Brown and Y. Chang. 2006. Growth, differentiation and malignant transformation of pre-B cells mediated by inducible activation of v-Abl oncogene. J. Immunol. 176:6831-6838.
Bogani, F. E. McConnell, L. Joshi, Y. Chang, G. Ghirlanda. 2006. A designed glycoprotein analogue of Gc-MAF exhibits native-like phagocytic activity. J. Am. Chem. Soc. 128:7142-7143.
Chen, Y., T. Fu. T. Tao, J. Yang, Y. Chang, M. Wang, L. Kim, L. Qu, J. Cassady, R. Scalzo & X. Wang. 2005. Macrophage activating effects of new alkamides from the roots of Echinacea species. J. Nat. Prod. 68: 773-776.
Pettit, G. R., H. Hoffman, H. D. L Herald, P. M. Blumberg, E. Hamel, J. M. Schmidt, Y. Chang, R. K. Pettit, N. E. Lewin and L. V. Pearce. 2004. Antineoplastic agents. 499. Synthesis of hystatin 2 and related 1H-benzo[de][1,6]-naphthyridinium salts from aaptamine. J. Med. Chem. 47: 1775-1782.
Jacobson, E., T. Beach, Y. Shen, R. Li, & Y. Chang. 2004. Reduction of the Mre11 DNA repair complex in Alzheimer´s Diseases. Mol. Brain Res. 128:1-7
Martina, C., A. Bell, J. Wayne and Y. Chang. 2003. In vivo ligation of CD3 on neonatal scid thymocytes blocks g-irradiation-induced TCR? rearrangements and thymic lymphomagensis. Immunol. Letter. 85: 279-286.
Perkins, E. J., A. Nair, D. O. Cowley, T. Van Dyke, Y. Chang and D. A. Ramsden. 2002. Sensing of intermediates in V(D)J recombination by ATM. Genes & Dev. 16:159-164.
Brown, M. L., D. Franco, A. Bürkel and Y. Chang. 2002. Role of PARP in DNA- PK independent recombination. Proc. Natl. Acad. Sci. USA 99:4532-4537.
Middlebrook AJ, C. Martina, Y. Chang, R. J. Lukas & D. DeLuca. 2002. Effects of nicotine exposure on T cell development in fetal thymus organ culture: arrest of T cell maturation. J Immunol. 169:2915-2924.
Brown, M. L., S. Lew and Y. Chang. 2000. The scid recombination-inducible cell line: a model to study DNA-PK-independent V(D)J recombination. Immunol. Letter. 75: 21
Brown, M. L and Y. Chang. 2000. Metabolism of recombination coding ends in scid cells. J. Immunol. 164:4135-4142.
Lew, S., D. Franco and Y. Chang. 2000. Activation of V(D)J recombination induces the formation of interlocus joints and hybrid joints in scid pre-B cell lines. Mol. Cell. Biol. 20(19):7170-7.
Brown, M. L., S. Lew, Y. Chang 1999. Coding end resolution in scid recombination-inducible cell lines. Gene Therapy and Molecular Biology Vol. 4:405-410.
Bosma, G.C., Y. Chang, and M. J. Bosma, 1999. Differential effect of an immunoglobulin m transgene on development of pre-B cells in fetal and adult scid mice. Proc. Natl. Acad. Sci. USA 96:11952-11957.
Chang, Y., M. J. Bosma and G. C. Bosma. 1999. Extended duration of DH-JH rearrangement in immunoglobulin heavy chain transgenic mice: implications for regulation of allelic exclusion. J. Exp. Med. 189: 1295-1305.
Chang, Y., and Brown, M. L. 1999. Formation of coding joints in V(D)J recombination-inducible scid pre-B cell lines. . Proc. Natl. Acad. Sci. USA 96: 191-196.
Chang, Y. and M. J. Bosma. 1997. Immunoglobulin transgenic scid mice: requirements for the rescue of scid B cell differentiation. Int. Immunol. 9:373-380.