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Charles Arntzen

Biography

Charles Arntzen studies plant-derived pharmaceuticals and vaccines in collaboration with faculty, government researchers, and industrial product development groups. He has used both transgenic and viral vector approaches to move genes into plant cells. His initial efforts focused on traits that improved crop production, which led him toward manufacturing protein pharmaceuticals in plant leaves. He and his collaborators pioneered subunit vaccine production and formulation.

Currently, Arntzen uses his experience in plant biotechnology in order to advise the National Academy of Sciences, other government agencies, and corporate entities on the strategic useof new technology in agriculture and human health programs. Though he isn't expanding his research team at ASU, he continues to grow his industrial and government collaborations and to support multi-disciplinary academic research teams.

 

Education

PhD, 1970, Purdue University, Lafayette, Indiana

 

Publications

  • Herbst-Kralovetz MM, Radtke AL, Lay MK, Hjelm BE, Bolick AN, Sarker SS,Atmar RL, Kingsley DH, Arntzen CJ, Estes MK, Nickerson CA. Lack of norovirus replication and histo-blood group antigen expression in 3-dimensional intestinal epithelial cells. Emerg Infect Dis. 2013 Mar;19(3):431-8.doi: 10.3201/eid1903.121029.
  • Velasquez LS, Shira S, Berta AN, Kilbourne J, Medi BM, Tizard I, Ni Y, Arntzen CJ, Herbst-Kralovetz MM (2011) Intranasal delivery of Norwalk virus-like particles formulated in an in situ gelling, dry powder vaccine. Vaccine 29: 32,5221-31
  • Phoolcharoen W, Dye JM, Kilbourne J, Piensook K, Pratt WD, Arntzen CJ, ChenQ, Mason HS, Herbst-Kralovetz MM. (2011) A non-replicating subunit vaccine protects mice against lethal Ebola virus challenge. ProcNatlAcadSci U SA. 2011 Dec 20;108(51):20695-700. Epub 2011 Dec 5. PMID:22143779
  • Huang Z, Phoolcharoen W, Lai H, Piensook K, Cardineau G, Zeitlin L, Whaley KJ, Arntzen CJ, Mason HS, Chen Q (2010) High-level rapid production of full-size monoclonal antibodies in plants by a single-vector DNA replicon system.,Biotechnology and Bioengineering, 106 (1), 9-17
  • Santi L, Batchelor L, Huang Z, Hjelm B, Kilbourne J, Arntzen CJ, Chen Q,Mason HS (2008) An efficient plant viral expression system generating orally immunogenic Norwalk virus-like particles., Vaccine, 26 (15), 1846-54

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  • L Santi, A Giritch, C Roy, S Marillonnet, V Klimyuk, Y Gleba, R Webb, Charles Arntzen, H Mason. Protection conferred by recombinant Yersinia pestis antigens produced by a rapid and highly scalable plant expression system. Proc Natl Acad Sci USA (2006).
  • Santi, L, Giritch, A, Roy, C, Marillonnet, S, Klimyuk, V, Gleba, Y, Webb, R, Arntzen, Charles, Mason, H. Protection conferred by recombinant Yersinia pestis antigens produced by a rapid and highly scalable plant expression system. (2006).
  • Charles Arntzen, Stanley Plotkin, Betty Dodet. Plant-derived vaccines and antibodies: Potential and Limitations. Vaccine (2005).
  • J Bryan, B Maloney, Naokazu Takeda, Yuriko Suzaki, Ami Yasushi, Tian Li, Tatsuo Miyamura, Charles Arntzen, Hugh Mason. Challenges in creating a vaccine to prevent hepatitis E. Vaccine (2005).
  • Y Thanavala, M Mahoney, S Pal, A Scott, L Richter, N Natarajan, P Goodwin, Charles Arntzen, H Mason. Immunogenicity in humans of an edible vaccine for hepatitis B. Proc. Natl. Acad. Sci., U.S (2005).
  • Z Huang, L Santi, K LePore, J Kilbourne, Charles Arntzen, H Mason. Rapid, high-level production of hepatitis B core antigen in plant leaf and its immunogenicity in mice. Vaccine (2005).
  • Thanavala, Y, Mahoney, M, Pal, S, Scott, A, Richter, L, Natarajan, N, Goodwin, P, Arntzen, Charles, Mason, H. Immunogenicity in humans of an edible vaccine for hepatitis B. (2005).
  • Thanavala, Y, Maoney, M, PaL, S, Scott, A, Richter, L, Natarajan, N, Goodwin, P, Arntzen, Charles, Mason, H. Immunogenicity in Humans of an Edible Vacine for Hepatitis B. (2005).
  • M Rigano, M Alvarez, J Pinkhasov, Y Jin, F Sala, Charles Arntzen, A Walmsley. Production of a fusion protein consisting of the enterotoxigenic Escherichia coli heat-labile toxin B subunit and a tuberculosis antigen in Arabadopsis thaliana. Plant Cell Rep (2004).
  • N Matoba, A Magerus, B Geyer, Y Zhang, M Muralidharan, A Alfsen, Charles Arntzen, M Bomsel, T Mor. A mucosally targeted subunit vaccine candidate eliciting HIV-1 transcytosis-blocking Abs. Proc Natl Acad Sci USA (2004).
  • R Peterson, Charles Arntzen. On risk and plant-based pharmaceuticals. TRENDS in Biotechnology (2004).
  • Mason, H S (Author) ,Chikwamba, R (Author) ,Santi, L (Author) ,Mahoney, R T (Author) ,Arntzen, Charles (Author) . Antigen delivery systems: Transgenic plants for mucosal vaccines. Mucosal Immunology (2004).
  • Mor, T S (Author) ,Mason, H S (Author) ,Kirk, D D (Author) ,Arntzen, Charles (Author) ,Cardineau, G A (Author) . Plants as Production and Delivery Vehicle for Orally Delivered Subunit Vaccines. New Generation Vaccines (2004).
  • Arntzen, Charles, Pitman, S. Biotechnology. Encyclopedia of Food and Culture (2003).
  • A Walmsley, Charles Arntzen. Plant cell factories and mucosal vaccines. Current Opinions in Biotechnology (2003).
  • A Walmsley, M Alvarez, Y Jin, D Kirk, S Lee, J Pinkhasov, M Rigano, Charles Arntzen, H Mason. Expression of the B subunit of Escherichia coli heat-labile enterotoxin as a fusion protein in transgenic tomato. Plant Cell Rep (2003).
  • Charles Arntzen, A Coglan, B Johnson, J Peacock, M Rodemeyer. GM Crops: Science, Politics and Communication. Nature Reviews Genetics (2003).
  • M Rigano, F Sala, Charles Arntzen, A Walmsley. Targeting of plant-derived vaccine antigens to immunoresponsive mucosal sites. Vaccine (2003).
  • M Smith, L Ricther, Charles Arntzen, M Shuler, H Mason. Structral characterization of plant-derived hepatitis B surface antigen employed in oral immunization studies. Vaccine (2003).
  • H Mason, H Warzecha, T Mor, Charles Arntzen. Edible plant vaccines: applications for prophylactic and therapeutic molecular medicine. TRENDS in Molecular Medicine (2002).
  • T Ganapathi, V Haridas, J Gutterman, Charles Arntzen. Metabolomics of plant saponins; Bioprospecting triterpene glycoside diversity with respect to mammalian cell targets. OMICS: Journal of Integrative Biology (2002).
  • Charles Arntzen. Agricultural biotechnology. J. Sci. Food Agric (2001).
  • Charles Arntzen, I Chalmers. The risks and rewards of biotechnology. Gastronomica (2001).
  • M Hanausek, P Ganesh, Z Walaszek, Charles Arntzen, T Slaga, J Gutterman. Avicins, a family of triterpenoid saponins from Acacia victoriae (Bentham), suppress H-ras mutations and aneuploidy in a murine skin carcinogenesis model. Proc. Natl. Acad. Sci. USA (2001).
  • Q Kong, L Richter, Y Yang, Charles Arntzen, H Mason, Y Thanavala. Oral Immunization with hepatitis B antigen expressed in transgenic plants. Proc. Natl. Acad. Sci. USA (2001).
  • T Ganapathi, N Higgs, P Balint-Kurti, Charles Arntzen, G May, J Van Eck. Agrobacterium-mediated transformation of embryogenic cell suspensions of the banana cultivar Rasthali (AAB). Plant Cell Reports (2001).
  • T Mor, M Sternfeld, H Soreq, Charles Arntzen, H Mason. Expression of recombinant human acetylcholinesterase in transgenic tomato plants. Biotechnology and Bioengineering (2001).
  • V Haridas, Charles Arntzen, J Gutterman. Avicins, a family of triterpenoid saponins from Acacia victoriae (Betham), inhibit activation of nuclear factor-B by inhibiting both its nuclear localization and ability to bind DNA. Proc. Natl. Acad. Sci. USA (2001).
  • V Haridas, M Higuchi, G Jayatilake, D Bailey, K Mujoo, M Blake, Charles Arntzen, J Gutterman. Avicins: Triterpenoid saponins from Acacia victoriae (Bentham) induce apoptosis by mitochondrial perturbation. Proc. Natl. Acad. Sci. USA (2001).
  • Hanausek, M, Ganesh, P, Walaszek, Z, Arntzen, Charles, Slaga, T, Gutterman, J. Avicins, a family of triterpenoid saponins from Acacia victoriae (Bentham), suppress H-ras mutations and aneuploidy in a murine skin carcinogenesis model. (2001).
  • Haridas, V, Arntzen, Charles, Gutterman, J. Avicins, a family of triterpenoid saponins from Acacia victoriae (Betham), inhibit activation of nuclear factor-?B by inhibiting both its nuclear localization and ability to bind DNA. (2001).
  • Haridas, V, Higuchi, M, Jayatilake, G, Bailey, D, Mujoo, K, Blake, M, Arntzen, Charles, Gutterman, J. Avicins: Triterpenoid saponins from Acacia victoriae (Bentham) induce apoptosis by mitochondrial perturbation. (2001).
  • Kong, Q, Richter, L, Yang, Y, Arntzen, Charles, Mason, H, Thanavala, Y. Oral Immunization with hepatitis B antigen expressed in transgenic plants. (2001).
  • A Walmsley, Charles Arntzen. Plants for delivery of edible vaccines. Current Opinion in Biotechnology (2000).
  • C Tacket, H Mason, G Losonsky, M Estes, M Levine, Charles Arntzen. Human immune responses to a Novel Norwalk virus vaccine delivered in transgenic potatoes. The Journal of Infectious Diseases (2000).
  • Charles Arntzen. Agricultural biotechnology. SCN News: Nutrition and Agriculture (2000).
  • Charles Arntzen. Plant-Derived Oral Vaccines: From Concept to Clinical trials In: International Seminar on Nuclear War and Planetary Emergencies 25th Session. World Scientific Publishing Co,Singapore (2000).
  • Charles Arntzen, D Kirk. Vaccination. McGraw-Hill Yearbook of Science & Technology (2000).
  • L Richter, Y Thanavala, Charles Arntzen, H Mason. Production of hepatitis B surface antigen in transgenic plants for oral immunization. Nature Biotechnology (2000).
  • N Bosque-Pérez, G May, Charles Arntzen. Applicability of an Agrobacterium-based system for the transformation of Musa species with diverse genomic constitution and ploidy level. Acta Horticulturae (2000).
  • Arntzen, Charles (Author) ,Kirk, D D (Author) . Vaccination. McGraw-Hill Yearbook of Science & Technology (2000).
  • Arntzen, Charles. Plant-Derived Oral Vaccines: From Concept to Clinical trials. World Scientific Publishing Co (2000).

Research Activity

Courses

Spring 2014
Course Number Course Title
BIO 495 Undergraduate Research
Fall 2013
Course Number Course Title
BIO 495 Undergraduate Research

Presentations

  • Matoba, Nobuyuki, Mittman, M., Griffin, T., Cherni, Irene, Arntzen, Charles, Mor, Tsafrir. Expression of an HIV-1 vaccine candidate expressed in N. benthamiana plan. PBVA 2: Plant Expression Systems for Recombinant Pharmacologic (Jun 2007).
  • Arntzen, Charles, Mahoney, R. Plant-derived vaccines: A new approach to international public health. 12th World Congress of Food Science, Chicago, IL (Jul 2003).
  • Herbst-Kralovetz, Melissa, Hjelm, Brooke, LaVigne, Daaimah, Kilbourne, Jacquelyn, Arntzen, Charles, Chen, Qiang, Mason, Hugh. Utilizing TLR Agonists to Augment Mucosal Vaccination. Keystone Symposia in Immunologic Memory

Service

  • Overcoming Global Vaccine Constraints with Heat-stable, Oral Vaccines Derived from GM Plants, Invited Speaker (2005 - Present)
  • National Center for Genome Resources, Member Board of Directors (2005 - Present)
  • Rockefeller Foundation - Blue Ribbon Panel on Vaccine Development, Co-Organizer & Participant (2004 - Present)
  • International Workshop on Advancing Technologies: Surveying the Global Capacity for Research, Invited Participant (2004 - Present)
  • ASU Mexico Joint Initiatives, Participant (2004 - Present)
  • Annual Meeting of the American Society of Plant Biologists, Speaker (2004 - Present)
  • Latin American Meeting on Agri. Biotec - Title: Plant-Made Pharmaceutical; From Basic Res. to Clinical Trials of Oral Vaccines, Speaker (2004 - Present)
  • Inauguration of Forestry and Agricultural Biotechnology Institute (FABI), Keynote Speaker (2004 - Present)
  • National Symposium: Basic Aspects of Vaccines - Title: Plant Derived Vaccines: Technology and Design, Plenary Speaker (2004 - Present)
  • ASU Associated Students for Biotechnology, Invited Speaker (2004 - Present)
  • Keystone Symposium on Rational Design of Vaccines and Immunotherapeutics, Speaker (2004 - Present)
  • National Nanotechnology and Nanoscience Advisory Board, Member (2004 - Present)
  • Advanced BioNutrition Corporation, Board of Directors and Scientific Advisory Board (2002 - Present)
  • Arizona State University, Member Academic Council (2002 - Present)
  • Center for the Study of Law, Science, and Technology at the ASU College of Law, Advisory Board (2002 - Present)
  • President George W. Bush's Council of Advisors on Science and Technology, Member (2001 - Present)
  • Burrill and Company Nutraceutical Capital Fund, Advisory Board (2000 - Present)
  • Council for Biotechnology Information, Distinguished Advisor (2000 - Present)
  • National Research Council's Review Committee for the Biomedical Research and Technology Transfer Partnership Awards Program of the State of Ohio, Chair (2002 - 2006)
  • Boyce Thompson Southwestern Arboretum, Board of Directors (2003 - 2004)
  • Burrill and Company Agbio Capital Fund, Advisory Board (2003 - 2004)
  • Conference on Plant Derived Vaccines and Antibodies: Potential and Limitations, Organizer and Speaker (2004)
  • Mesa Community College, Advisory Board (2004)
  • NIH Center for Sexually Transmitted Infections & Topical Microbicides Cooperative Research Center, Director (2004)
  • Nanoscience and Nanotechnology Advisory Board, Member (2004)
  • SOLS Director Search Committee, Member (2004)
  • Targeted Recruitment Committee, Organizer (2004)
  • US State Department Title: Perspectives on the Future of Science and Technology, Invited Speaker (2004)
  • Economic Development Breakfast, Speaker (2003)
  • Epicyte Pharmaceutical Inc, Scientific Advisory Board (2003)
  • Third Wave AgBio, Inc, Board of Directors (1998 - 2001)
  • Kimeragen, Inc, Board of Scientific Advisors (1996 - 2001)
  • Integrated Protein Technologies Scientific , Monsanto, Advistory Board (1999 - 2000)
  • Boyce Thompson Institute for Plant Research, Ithaca, New York, President/CEO (1995 - 2000)